The potato (Solanum tuberosum L.), cultivated in Poland, Europe, and all over the world, varies in shape, size, and also in the colour of the skin and flesh - from white, through yellow and red to dark purple, depending on the variety. Coloured tubers have an attractive colour and a high nutritional value due to their mineral content, which plays an important role in terms of agriculture as well as health. The nutritional value of potato tubers is influenced by many different factors, including variety, growing conditions, and climatic conditions. An analysis of these is reasonable in order to manage the crop effectively. The aim of this study was to compare the content and uptake with tuber yield of selected macronutrients (nitrogen, calcium and phosphorus) in potato tubers of different flesh colours: Eurostar (light yellow), Rote Emma and Herbie 26 (red flesh), Provita, Salad Blue, Blaue Annelise, Vitelotte Noire (purple flesh) and Bora Valley (dark purple flesh) in three growing seasons from 2021-2023. The content of selected elements was analyzed using Inductively Coupled Plasma - Optical Emission Spectrometry (ICP-OES). Macronutrients were quantified by reference to the calibration curve of a multi-element standard solution Standard in its linear range. Macronutrient intake was calculated as the product of the potato tuber dry matter yield and the content of each element. Potato varieties with red and purple flesh accumulated similar or higher amounts of selected macronutrients (nitrogen, calcium and phosphorus) than those with light flesh. The content and uptake of the analyzed macronutrients were influenced by weather conditions during the study years.