The aim of this study was to investigate cadmium (Cd) levels in wild bats in Poland. Concentration levels of Cd were studied in the guano samples collected from 4 bat summer (nursery) colonies of the wildlife greater mouse-eared bats (Myotis myotis) located in various parts of Poland. Analysis was made using inductively coupled plasma optical emission spectrometry (ICP-OES) method. During present investigation Cd was noted in all colonies and in all samples included into the study. Cd concentration levels fluctuated from 1.06 mg kg-1 to 3.54 mg kg-1 (median value 1.905 mg kg-1). Clear differences in Cd concentration levels were noted between particular bat colonies. Moreover, the present study have shown that guano samples analysis is a suitable method to evaluate of Cd concentration levels in wild bats. The results show that wildlife greater mouse-eared bats in Poland are exposed to Cd. Due to high toxicity of Cd even in small doses,  it can be assumed that this metal may be a factor influencing the health status of wild bats in Poland