The research material consisted of table potato tubers harvested in a three-year field experiment carried out in 2018-2020 at the Agricultural Experimental Station in Zawady of University of Siedlce in triplicate on tawny soil. The main factor was two mid-early potato cultivars, Oberon and Malaga, and the sub-plot factor included five options of treatment with herbicides and biostimulants (1. control, 2. the herbicide Avatar 293 ZC, 3. the herbicide Avatar 293 ZC and the biostimulant PlonoStart, 4. the herbicide Avatar 293 ZC and the biostimulant Aminiolant, 5. the herbicide Avatar 293 ZC and the biostimulant Agro-Sorb Folium). The objective of the study was to determine the impact of biostimulant and herbicide applications on the content and uptake of selected micronutrients by potato tubers. Manganese (Mn), and copper (Cu) contents were determined in tuber dry matter using an ICP-OES spectrometer (Perkin Elmer, Optima 8300, USA). Higher Cu contents were found in cv. Malaga tubers compared with Oberon, while greater uptake of these elements (Mn, Cu) with tuber yield was determined in cv. Oberon due to its higher yield. An application of biostimulants increased Cu contents as well as Mn, and Cu uptake compared with control tubers. The most beneficial effect was produced by the using of the biostimulant Agro-Sorb Folium. Manganese and copper were significantly affected by the weather during the study years. It should be added that recently, agriculture has become increasingly aware of the environmental impact of the measures used, and in this context, biostimulants are gaining popularity to improve the quality of crops, minimizing the negative impact on the environment.