Inadequate or suboptimal intake of zinc (Zn) can lead to hidden health problems that affect a significant portion of the global population, especially women living in impoverished regions with a high risk of malnutrition, including young children. Therefore, the enrichment of Zn in edible plant tissues through Zn fertilization is considered an effective approach to prevent health problems associated with nutrient deficiencies. This study aims to investigate the biological enrichment of Zn in some microgreens belonging to the Brassicaceae family during the production process and evaluate their Zn uptake potential. Cauliflower, broccoli, and cabbage microgreens grown with the addition of different doses of ZnSO4 (0, 5, 10, 20 mg l-1) in the nutrient solution were analyzed for their fresh and dry weights, as well as nutrient element contents (P, K, Ca, Mg, Fe, Mn, Zn, and Cu). It was found that increasing Zn levels had different effects among the varieties, and the response to Zn enrichment was genotype-specific. The highest fresh weight (13.89 g) was observed in broccoli microgreens with 10 mg l-1 Zn application, while the lowest fresh weight (5.87 g) was found in cauliflower microgreens with the control treatment (Zn0). The highest Zn content was determined in cauliflower, broccoli, and cabbage microgreens treated with 20 mg l-1 Zn. Additionally, the application of 5 mg l-1 Zn significantly affected the Ca, Mg, and Mn contents of cabbage microgreens. The biological enrichment of Zn in cauliflower, broccoli, and cabbage microgreens from the Brassicaceae family is expected to have positive effects on human health and can be evaluated, particularly for improving Zn deficiency.