The aim of the study was to evaluate the effect of nitrogen (N) and sulphur (S) fertilizer on the selenium content and selenium (Se) uptake in grain DM of spring wheat. A field experiment (2009-2011) was conducted on Cambisols (WRB 2007) in south-eastern Poland. The experiment included 2 variables: N fertilization (0, 40, 80, and 120 kg ha-1) and S fertilization (0 and 50 kg ha-1). The experiment showed a positive effect of nitrogen and sulphur fertilization on grain yield of spring wheat cv. Tybalt, with the highest yield obtained after the application of 80 kg N ha-1 (5.40 t ha-1) and 120 kg N ha-1 (5.59 t ha-1), which resulted in an average increase of 1.42 t ha-1 (34.7%) with respect to the control. S fertilization increased grain yield by 3.58%. the mean selenium  content in the spring wheat grain was 0.085 mg kg-1 and the selenium uptake equalled 0.419 g ha-1. The selenium content and uptake by grain DM increased significantly following the application of N at doses of 40 and 80 kg ha-1: the content rose by 19.1% and 36.8%, respectively, and the uptake was 24.4% and 84.7% higher than in the control. Following the application of the nitrogen dose of 120 kg ha-1 no further statistically significant increase in the content and uptake of selenium in comparison with the application of the nitrogen dose of 80 kg ha-1. The content of selenium and uptake of selenium by the grain following the application of sulphur at a dose of 50 kg ha-1 increased by 20.8% and 25.3%, respectively, in comparison with the control. A positive correlation was found between the content of selenium in grain and the selenium uptake by grain DM (r = 0.947).

22.3.2017