In a two-factor pot experiment, which was conducted 2004-2005, the direct and successive impact was estimated of mixed application of different doses of municipal sewage sludge (0.5, 1.0, 1.5 and 2.0% d.m. of sewage sludge relative to 6 kg d.m. soil in pot) and a constant dose of wheat straw (30 g d.m. per pot), with and without supplemental mineral fertilization with nitrogen and NPK, on the content, uptake and utilization of copper, manganese and zinc by test plants. The soil used in the experiment was brown acid incomplete soil (good rye complex) and the test plant in the first year of research was grass – Festulolium, which was harvested four times, and in the second year – common sunflower and blue phacelia. In mean object samples of Festulolium, common sunflower and phacelia, content of copper, manganese and zinc was marked with the ASA method after mineralization in a mixture of nitric(V) and perchloric acid(VII). Rising doses of municipal sewage sludge with addition of a fixed dose of wheat straw, both in direct and successive effect, increased the content of copper, manganese and zinc in test plants. The increase in the weighted mean (from four swaths) content of copper in Festulolium, in comparison with the control object, varied from 8.04 to 59.8%, manganese from 21.8 to 68.8% and zinc from 19.4 to 59.1%. In the second year, the mean increase in the content of copper in common sunflower from objects fertilized with sewage sludge and straw varied from 8.7 to 30.3% and in phacelia from 6.1 to 12.6%. By analogy, the mean content of manganese rose from 23.3 to 59.5% and from 5.9 to 33.1% and the content of zinc from 33.2 to 50.3% and from 15.9 to 37.9%. Mineral fertilization with N and NPK,increased the mean content of all microelements in test plants, with the increase being larger after NPK than N fertilization. The uptake of microelements by plants from sewage sludge and straw, in most cases, was increasing along with the increase of the doses of sewage sludge. In the total uptake of individual microelements, about 2/3 were atken up by Festulolium and the remaining 1/3 by phacelia. Utilization of individual microelements from sewage sludge and straw was considerably diverse. IIn the two years, test plants utilized manganese mostly (on average 58.2%), less zinc (on average 5.54%) and to the smallest degree copper (on average 3.03%).