In the third year of a pot experiment (in a greenhouse), the carryover influence of fertilization with fresh sewage sludge and, for comparison, mineral fertilization on the content and uptake of Fe, Mn, Mo, B, Ba, Sr, As, Sn, Li, and Ti by the biomass of Miscanthus sacchariflorus grass. The yield of silver-grass biomass was evaluated on the basis of two harvests (June and December). Trace elements in the tested grass as well as in the soil (after harvest) were determined by means of the ICP-AES technique after sample combustion in a muffle furnace. Significant influence of sewage sludge fertilization on yield of silver-grass biomass was found. Grass harvested in autumn contained higher contents of some analyzed trace elements (Fe, Mn, Sr, Ba, and Ti) than that harvested in summer. The uptake of trace elements uptake by the silver-grass biomass was higher from the objects fertilized with sewage sludge than the ones receiving mineral fertilization. After three years of cultivation, the soil contained more Fe, Mn, Ba, Sr, Ti, B, and Li as compared to the content before the experiment.