Otrzymano: Styczeń 13, 2018
Zaakceptowano: Kwiecień 16, 2018
Opublikowano online: 2018-06-11
Biology and microbiology,
Pollution and environment
The research objective has been to determine the effectiveness of bioaugmentation of soil polluted with diesel oil VERVA (DO) in restoring the biological balance of the contaminated soil. Another goal was to determine the suitability of bioaugmentation for degradation of petrols (C6-C12), mineral oils (C12-C35), volatile hydrocarbons (BETX) and ∑9 polycyclic aromatic hydrocarbons (PAHs) present in diesel oil. The research included a pot experiment. Two soils (loamy sand and sandy loam) were tested, either unpolluted or polluted with DO in a dose of 7 cm3 kg-1. Maize served as a test plant. Bioaugmentation was carried out with the biopreparation BIO ACTIV HGS code 208 (BC) and with a consortium of bacteria (CN) isolated from soil contaiminated with diesel oil.It has been found that the extent of DO toxic effects on plants depended on the textural composition of soil. Diesel oil was more toxic to maize grown on loamy sand than on sandy loam. The pollutant disturbed proportions between fast-growing (strategy r) and slow-growing (strategy K) microorganisms. It had an adverse effect on the ecophysiological diversity of microorganisms. Bioaugmentation of DO-polluted soil changed proportions in the structure of bacterial communities. There was a partial shift of the assemblage of actynomycetes towards strategy r while organotrophic bacteria shifted towards strategy K. Bioaugmentation alleviated the negative influence of DO on the diversity of microorganisms, and on the growth and development of maize. It accelerated degaration of petrols and mineral oils.
Borowik A., Wyszkowska J. 2018. Bioaugmentation of soil contaminated with diesel oil. J. Elem., 23(4): 1161-1178. DOI: 10.5601/jelem.2018.23.1.1627
diesel oil, bioaugmentation, soil microbiome, degradation of hydrocarbons