The study assessed the effect of various forms of sulphur (Na2S2O3, elemental S and Na2SO4) and nitrogen (UAN-30, NH4NO3) on calcium and magnesium content and uptake in spring wheat and cocksfoot. A two-year pot experiment was conducted on soil material of clayey silt granulometric composition. Before the experiment, the soil was characterized by slight acidity and low content of assimilable forms of phosphorus, potassium, magnesium and sulphur. The results show that the experimental factors caused noticeable variation in the content and uptake of calcium and magnesium in spring wheat and cocksfoot. Among the sulphur fertilizers, the greatest increase in calcium and magnesium content and uptake was produced by application of sodium sulphate. The increase in calcium and magnesium uptake was more marked in the case of plants fertilized with nitrogen in the form of ammonium nitrate. The effect of nitrogen fertilization on the analyzed parameters also depended on the species of plant. Wheat grown in the series with ammonium nitrate was characterized by higher Ca and Mg content and uptake than plants fertilized with nitrogen in the form of liquid UAN 30. Calcium uptake by wheat fertilized with NH4NO3 was on average 15% higher for grain and 9% higher for straw compared to wheat fertilized with UAN 30. As for magnesium, the increase was 24.5% and 18%, respectively. Concerning cocksfoot, fertilization with UAN 30 had a greater affect on content and uptake of the analyzed nutrients than ammonium nitrate.