Otrzymano: Czerwiec 12, 2017
Zaakceptowano: Październik 10, 2017
Opublikowano online: 2018-02-26
Biology and microbiology,
Medicine and veterinary
The aim of this research was to evaluate, on an animal model, the influence of a change in a diet composition and dietary supplementation (complementary or excessive) with vitamins B1, B2, B6 and niacin on the selenium content and antioxidant enzymatic defence of the testicular tissue. Forty-four male Wistar rats were divided into four equal groups; the control group (I) was fed Basic Diet (BD), while groups II-IV were given Modified Diet (MD), in which 83.5% of wheat was replaced with wheat flour and 50% of maize was replaced with sucrose. Animals from groups I-II received only water to drink, while group III was given an aqueous solution of vitamins supplementing the deficiency created by the dietary modification (MD + Adequate Supplementation: B1-0.94, B2-0.48, B6-0.5, niacin-1.9 mg); group IV received a solution in order to supplement both the deficiency resulting from the change in a diet and the recommended prophylactic dose of vitamins (MD + Excessive Supplementation: B1-3.1, B2-2.3, B6-2.4, niacin-6.65 mg). Blood serum was examined to determine glucose and selenium concentrations. The activity of GPx, CAT, SOD, GST and selenium content were measured in the testicular tissue. The change in a diet composition made by replacing full grains with wheat flour and sucrose and dietary supplementation with B1, B2, B6 vitamins and niacin with varying amounts of feed (p ≤ 0.01), selenium (p ≤ 0.01) and liquids (p ≤ 0.01) did not significantly influence the body gain of the animals, glucose concentration in blood or the selenium content and activity of antioxidant defence enzymes in testicles. A significant (p ≤ 0.05) decrease in the selenium concentration in the blood of animals supplemented excessive amounts of the above vitamins, with a lack of significant differences in the selenium content in testicles, co-occurred with the highest activity of antioxidant enzymes.
Goluch-Koniuszy Z., Drozd R., Kołodziejski M. 2018. Selenium status of serum and testicles of male rats fed a diet containing sucrose and supplemented with chosen vitamins from the B group. J. Elem., 23(2): 659 - 671. DOI: 10.5601/jelem.2017.22.3.1474
testis tissue, blood, antioxidant defense, Se, vitamin