In order to evaluate a potential threat to soil environment by heavy metals, it is important to separate and determine their fractions by means of sequential extraction. The research aimed at evaluating the total content of lead and chromium as well as their fractions in soils localized on a moraine slope in the Siedlce Heights (transects A and B). Sequential fractionation of these elements was carried out according to Zeien and Brümmer’s method. Chemical analyses revealed varied contents of seven lead and chromium fractions in the soils. The largest amounts of both heavy metals were mostly recorded in the residual fraction (F7), and those of lead also in the organic fraction (F4). The least lead was found in the exchangeable fraction (F2) (it was not detected in easily soluble fraction F1), while the least chromium appeared in the easily soluble (F1) and organic fractions (F4). Statistical processing revealed that the examined lead and chromium fractions generally depended on separated fractions, total contents of these metals, and some properties of analyzed soils.