Animal-model assessment of the impact of diet modification and vitamin B supplementation on the pericardial fatty tissue and its fatty acid profile
Sierpień 08, 2017
Styczeń 09, 2018
Goluch-Koniuszy Z., Domiszewski Z.
The objective of this research is to examine, on an animal model, the consequences of a diet modification where ingredients such as full wheat and maize grain have been isocalorically replaced with white flour and sucrose. This research also examines how dietary supplementation (adequate and excessive one) with synthetic B vitamins affects the amount of pericardial fatty tissue and its fatty acid profile. 48 male Wistar rats were divided into 4 groups: group I was fed Basic Diet (BD), while groups II-IV were given Modified Diet (MD), in which 83.5% of wheat was replaced with wheat flour and 50% of maize was replaced with sucrose. Animals from groups I-II received only water to drink, while group III was given an aqueous solution of vitamins supplementing the deficiency created by the dietary modification (MD+Adequate Supplementation); group IV received a solution in order to supplement both the deficiency resulting from the dietary modification and the recommended prophylactic dose of vitamins (MD+Excessive Supplementation). Subsequently, the blood serum was examined to determine the glucose level. The heart and pericardial fats were dissected and their content of fatty acids was determined by the GC method. It has been ascertained that MD led to a significant increase in feed and energy consumption but did not influence the increase in body mass, heart mass, pericardial fatty tissue, or glucose concentration in the blood of the animals. However, it did cause changes in its fatty acid profile, lowering the content of MUFA and increasing PUFA. The MD+AS supplementation did not yield the same effect. The MD+ES supplementation combined with the lowered consumption of feed caused an increase in glycaemia and changes in the profile of pericardial fatty tissue, similar to those observed in the control group.
Goluch-Koniuszy Z., Domiszewski Z. 2018. Animal-model assessment of the impact of diet modification and vitamin B supplementation on the pericardial fatty tissue and its fatty acid profile. J. Elem., 23(3): 849 - 862. DOI: 10.5601/jelem.2017.22.3.1514
supplementation, B group vitamins, pericardial fat, fatty acids