The main aim of the study, conducted in 2007-2008, was to define the influence of nitrogen levels 0, 50, 100, 150, 200 mg N dm-3 applied to grass lawns on the content of metallic microelements: iron, manganese, zinc and copper and on their quantitative ratios in turf grass. The study was carried out on a 2-year-old lawn, composed of a mixture of lawn grasses species and varieties: perennial ryegrass (Lolium perenne L.) var. Grasslands Nui (45%), tall fescue (Festuca arundinacea Schreb) Finelawn (25%), red fescue (Festuca rubra Hack.) Olivia (10%), red fescue (Festuca rubra Hack.) Boreal (15%) and Kentucky bluegrass (Poa pratensis L.) Balin (5%). With the exception of copper, the nitrogen fertilization raised the content of iron, manganese and zinc in aerial plant parts. The application of nitrogen (as ammonium nitrate) increased the uptake of nutrients by aerial plant parts: Fe to 143.0%, Mn – 227.2%, Zn – 233.3% Cu – 180.7%, and modified the ratios between amounts of the microelements. In general, it has been demonstrated that nitrogen nutrition of plants grown on ornamental lawns, within ranges of N – 150 to N – 200, increased the ratios of Fe : Mn : Zn : Cu. The best ornamental quality of lawns was obtained where the aerial plant parts contained (in mg kg–1 d.m.): Fe 231.6-292.8, Mn 35.6-50.5, Zn 26.5-47.5 and Cu 16.9-17.6. These ranges could be recommended as preliminary guidelines for ornamental lawns. In practice, considering the positive and stimulating influence of nitrogen nutrition on crops, the chemical composition of plants and the uptake of microelements by a mixture of grasses on ornamental lawns, it is crucial to monitor actual fertilization by taking into account losses of nutrients. These losses are caused by intensive uptake of nutrients by the aerial plant parts and due to the leaching of nutrients from the rhizosphere. It is necessary to replenish lost elements using mineral fertilizers.