The aim of the research was to determine the influence of foliar nutrition with Pentakeep V as well as differentiated nitrogen fertilisation on the content of macro- (Ca, K, Mg, Na, P and S) and micronutrients (B, Cu, Fe, Mn and Zn) in spinach leaves. Pentakep V is a fertilizer containing 5-aminolevulinic acid (5-ALA), which is the direct precursor of chlorophyll in plants. In 2006-2007, a pot experiment with spinach Spinacia oleracea L. cv. Spinaker F1 was carried out. The plants were cultivated in 60 × 40 × 20 cm containers placed in an open field under a shade-providing fabric. Containers were filled with loamy clay soil (35% of sand, 28% silt, 37% clay) with the organic matter content of 2.44% in 2006 and 2.52% in 2007. The experiment design included 2 sub-blocks: with and without foliar nutrition. The plants were sprayed twice with Pentakeep V fertilizer in a dose of 0.02% w/v (16 ml 100 dm–3 – 3000 dm3 per 1 ha). In each sub-block, soil fertilization with nitrogen was applied: 1 – control (without N fertilization), 2 – 25 mg N dm–3 of the soil (50% of N dose), 3 – 50 mg N dm–3 of the soil (100% of N dose). Nitrogen fertilization was applied in the form of ammonium nitrate prior to seed sowing. Among all of the determined nutrients, a significant interaction between foliar nutrition and soil application of nitrogen was observed in the case of Ca and Fe content in spinach leaves. Foliar application of Pentakeep V decreased the content of Ca in plants without N fertilization as well as increased the amount of this element in plants fertilized with full dose of nitrogen (100% of N dose). These observations were further verified by the changes of Ca content in soil after plant cultivation. Increased uptake of Ca from soil was observed for plants treated with Pentakeep V and fertilized with the full dose of nitrogen. A higher content of this element in soil was noted after cultivation of plants receiving only Pentakeep V (without N fertilization). Specific interaction of Pentakeep V on the increased content of Fe in spinach fertilized with 50% dose of N was observed. In comparison with the control, N fertilization in both doses (regardless of the foliar nutrition) led to the decrease of the plant content of Ca, Na and Fe as well as resulted in the increased concentration of K in spinach leaves. Plants fertilized with half-dose of N were characterized by lower content of Mn but plants treated with 100 % of nitrogen had higher concentration of this element in comparison to the control. Foliar application of Pentakeep V (considered independently of N fertilization) did not significantly influence the content of these nutrients in spinach leaves. Weather conditions throughout both years of cultivation had no effect on the interaction between foliar nutrition with Pentakeep V and N fertilization on the content of analyzed nutrients.