Skip to content
Journal Title Abbrev.
J. Elem.
ISSN – 1644-2296
DOI: 10.5601

Search article

flag flag
Applicability of biochemical indices to quality assessment of soil polluted with heavy metals

Issue: 4/2013

Recevied: Nov 04, 2013

Accepted: Nov 04, 2013

Published: 2014-01-06


Wyszkowska J., Borowik A., Kucharski J., Kucharski M.

Categories: Biology and microbiology, Pollution and environment

DOI: 10.5601/jelem.2013.18.4.504


The objective of this study has been to test a series of biochemical soil quality indices so as to select the one(s) that would mainly rely on enzymes participating in the processes of carbon and nitrogen transformations and reflect objectively the quality of soil. Moreover, an ideal index should be comparable to yields of crops. A vegetative experiment with 5 replications was set up on two soils: loamy sand and sandy loam, which had been polluted with cadmium, copper and zinc. Once the soil moisture content was raised to the level of 60% capillary water retention capacity, the following plants were sown: oat, spring oilseed rape and yellow lupine. Twice during the growing season, the activity of soil enzymes such as dehydrogenases, catalase, urease, ß-glucosidase, acid phosphatase, alkaline phosphatase and arylsulphatase was determined. Next, based on the soil enzymatic activity as well as the content of clay and organic carbon, 21 indices of the biochemical soil activity (BA1 to BA21) were proposed, which were divided into two groups: simple and complex indices. In addition, coefficients of correlation between yields of plants and biochemical soil quality indices were calculated. The experiment has demonstrated that the activity of enzymes should be expressed in units of the product of a catalyzed reaction in 1 h time calculated per 1 kg d.m. of soil, i.e. the activity of dehydrogenases in μmol TFF, catalase – mol O2, alkaline phosphatase and arylsulphatase – mmol PNP, while that of urease in mmol N-NH4+. Introduction of uniform units facilitates comparison of the results and quality assessment of different soils, irrespective of the author or research centre they originate from. It has also been shown that among the 21 tested biochemical indices of soil quality assessment, the best ones are BA20 = %C × (Deh + Pal + Pac + Ure), derived from the content of organic carbon and activity of four enzymes: dehydrogenase, alkaline phosphatase, acid phosphatase and urease, and BA21 = Deh + Kat + Pal + Pac + Ure + Glu + Aryl, calculated from the activity of seven enzymes: dehydrogenases (Deh), catalase (Cat), acid phosphatase (Pac), alkaline phosphatase (Pal), urease (Ure), ß-glucosidase (glu) and arylsulphatase (Aryl).


Wyszkowska J., Borowik A., Kucharski M., Kucharski J. 2013. Applicability of biochemical indices to quality assessment of soil polluted with heavy metals. J. Elem. 18(4): 733-756, DOI: 10.5601/jelem.2013.18.4.504


cadmium, copper, zinc, soil enzymes, biochemical index of soil quality

About issue:

Download article