Primary and secondary metabolites, which are natural products produced by plants, are mainly used directly or indirectly in industry. These are active substances that are predominantly used in plant metabolism (For example, etheric oils (essential oils, essences), alkaloids, tannins, bitter substances, etc.). They increase body's defence sysytem, support the functions of organs and accelerate healing. Thus, they have positive effects on the functions of certain tissues and organs. In this study, some bioactive and biochemical compounds, dry matter, total antioxidant activity, total flavonoid and total phenolic content, macro-micro and heavy metal content, essention oil yield and content in  Origanum onites and Origanum dubium species were determined. It was determined that total ash, dry matter, total antioxidant activity, total phenolik content, total flavonoid content, chlorophyll and NBI amount were higher in Origanum dubium than Origanum onites. Yield of essential oil was determined between 3.130 and 3.240 in Origanum onites and Origanum dubium species, respectively. Carvacrol (78.24%), γ-terpinene (6.650%) and Cymene (3.950%) components were found to be dominant in the essential oil of Origanum  onites. On the other hand, the main components of essential oil obtained from Origanum dubium species were Carvacrol (22.01%), γ-terpinene (23.55%), cis-sabinene hydrate (13.90%), Terpinen-4-ol (8.500%), α-terpinene (4,94%), Cymene (4,94%) and trans-sabinene hydrate (3,35%).