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Journal Title Abbrev.
J. Elem.
ISSN – 1644-2296
DOI: 10.5601

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Evaluation of serum and tissue magnesium, Vascular Endothelial Growth Factor and osteopontin levels in dogs with mammary tumor with/without pulmonary metastases and in healthy dogs

Issue: 1/2021

Recevied: Jun 02, 2020

Accepted: Dec 20, 2020

Published: February 11, 2021

Authors:

Günay Uçmak Z., Koenhemsi L., Uçmak M., Yalçın E., Gönül R., Ates A.

Categories: Medicine and veterinary

DOI: 10.5601/jelem.2020.25.4.2000

Abstract:

Mammary tumors in female dogs are usually malignant and tend to metastasize to distant organs, especially to regional lymph nodes and lungs. Radiography is the standard diagnostic method to detect pulmonary metastases in these animals. Magnesium (Mg), vascular endothelial growth factor (VEGF), and osteopontin (OPN) levels have been used in recent studies to make prognoses of human breast cancer. To the best of our knowledge, however, there are not many studies that have been performed on this subject, and there is no study on animals in which the three indicators are scrutinized together. The aim of this present study is to evaluate Mg, VEGF, and OPN levels in healthy dogs and in dogs with mammary tumors with/without pulmonary metastases, and to investigate the alterations of these parameters in the serum and tissue samples of dogs with mammary tumors in connection with the histological tumor type and tumor grade. Mammary tumor groups were designed according to the presence of pulmonary metastasis in radiography; group M1 consists of 20 dogs with metastatic mammary tumors to the lung, and group M0 consists of 20 dogs with nonmetastatic mammary tumors. Ten clinically healthy dogs composed group H. The dogs represented different breeds and ages. Three-view thoracic radiographs were taken to determine any metastasis in lungs by digital radiography. Magnesium, VEGF, and OPN were determined in the mammary gland samples and blood serum of 40 dogs with malignant mammary tumors and in 10 healthy dogs. The magnesium levels were measured by atomic absorption spectrophotometry, both in the tissue and serum samples. Also, the tissue and serum VEGF and OPN levels were determined by ELISA with commercially available kits. The tissue Mg levels in the M0 group were significantly (P

Citation:

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Uçmak Z.G., Koenhemsi L., Uçmak M., Yalçın E., Ateş A.,  Gönül R. 2021. Evaluation of serum and tissue magnesium, Vascular Endothelial Growth Factor and osteopontin levels in dogs with mammary tumor with/without pulmonary metastases and in healthy dogs. J. Elem., 26(1): 125 - 135. DOI:  10.5601/jelem.2020.25.4.2000

Keywords:

Canine mammary tumor, Magnesium, Osteopontin, Vascular endothelial growth factor

About issue:

26.1.2021
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