The objective of the study was to investigate the influence of pre-milking teat disinfection on total bacterial contamination of teat skin, and to analyze the effect of pre- and post-milking teat disinfection on somatic cell count in milk. Three groups of cows in five dairy farms were used. The total bacterial contamination on cow teat skin before and after teat disinfection was determined. The number of colony forming units (cfu) was calculated per 1 ml a total for 2700 samples. The most bacteria on teat skin were effectively reduced after pre-milking teats disinfectionm almost in all groups of 5 farms (from 1.1 to 4.5 times). Summarized results showed that the number of bacteria counted on teat skin and milk somatic cell count (SCC) were significantly higher from teats with no pre- and post-milking teat disinfection in comparison with teats with pre- and post-milking teat disinfection or no pre-milking teat disinfection and post-milking teat disinfection only (p≤0.05). Pre-milking teat disinfectant with peracetic acid and hydrogen peroxide was effective in reducing total bacterial contamination on cow teat skin. The results also indicate that application with pre- and post-milking teat disinfectants provided an impact on the reduction of SCC in milk. The lowest iodine content was 0.1291 mg L-1 (group T1, whereas the highest was 0.2963 mg L-1). In our research the differences in the iodine content between farms were noted as well. For example, the highest iodine content in milk appeared at FIV farm. Statistical differences appeared at p≤ 0.05.  

25.1.2020