Monitoring of changes occurring in the microbiota of soil contaminated with Ekodiesel ULTRA diesel oil (DO) is crucial for effective bioremediation. Therefore, the aim of this study has been to determine the functional diversity of bacterial communities, based on an analysis of  the metabolic profile in soil contaminated with diesel oil for 270 days. The rate of the degradation of volatile hydrocarbons (BETX) by soil microorganisms was determined. In the assessment, the activity of selected extracellular enzymes was also made.The study revealed that the contamination of soil with Ekodiesel Ultra diesel oil has a lasting effect on the soil environment, as certain groups of organic compounds included in the composition of DO are relatively resistant to biodegradation. They include gasoline fractions (C6-C12), mineral oil (C12-C35) and xylene isomers found in the composition of BTEX. Ekodiesel Ultra diesel oil increased the counts of most groups of microorganisms as well as raising the enzymatic activity of soil. The catabolic profile of the bacteria, determined on Biolog ECO MicroPlate® microplates, the eco-physiological profile of the bacteria (EP index) and the identification using MALDI-TOF mass spectrometry MS prove that DO causes changes in the structure of bacterial communities. Their diversity decreases and the mutual relationships between the r-strategy and K-strategy bacteria change. The methods applied durign the research ensured good identification of the eco-physiological and catabolic profile of bacteria, which would facilitate optimisation of the biodegradation of diesel oil.  

23.3.2018