Activity of β-glucosidase, arylsulfatase and phosphatases in soil contaminated with copper


Wydanie: 1 / 2010

Autorzy:

  • Wyszkowska J., Kucharski M., Kucharski J.

Kategorie: Agricultural , Biology and microbiology , Pollution and environment

DOI: 10.5601/jelem.2010.15.1.213-226

Abstrakt:

A  pot  experiment  was  carried  out  to  determine  the  effect  of  soil  (loamy  sand  and sandy loam) contamination with copper doses of 0, 150, 450 mg Cu⋅kg-1  d.m. soil on the activity of β-glucosidase (EC 3.2.1.21), acid phosphatase (EC 3.1.3.2), alkaline phosphatase (EC 3.1.3.1) and arylsulfatase (EC 3.1.6.1) in soil. The resistance of these enzymes to copper  pollution  was  also  estimated.  Soil  samples  were  contaminated  with  copper  chloride. The experiment was carried out in five replications, in two series. The first series was performed on uncropped soil and the second one – on cropped soil. The experimental plants were oat, spring rape and yellow lupine. The activity of soil enzymes was determined in the analyzed samples on the 25th and the 50th day of the experiment.The results of the experiment showed that copper contamination in doses of 150 mg to  450  mg⋅kg-1 soil  significantly  inhibits  soil’s  biochemical  activity.  The sensitivity  of the tested enzymes  to  copper  was  determined  in  the  following  order: alkaline phosphatase>arylsulfatase>acid phosphatase>β-glucosidase. The resistance of the above enzymes to copper depended on the cultivated plant species, soil type and the type of soil use and management. In samples of sandy loam, copper induced the smallest change in the activity of acid phosphatase and alkaline phosphatase, and in loamy sand – in the activity of arylsulfatase and acid phosphatase. In uncropped soil, copper was the least effective in changing the activity of arylsulfatase and acid pho- sphatase. All of the tested enzymes were less resistant to copper contamination in cropped than in uncropped soil. In soil planted with oat, β-glucosidase was the most resistant and arylsulfatase  was  the  least  resistant enzyme  to  copper contamination.  In samples  sown with spring rape, the analogous enzymes were arylsulfatase and alkaline phosphatase. In yellow  lupine treatments,  alkaline  phosphatase  was  the  most and  β-glucosidase was  the least resistant enzyme.


Cytacja:

Wyszkowska J., Kucharski M., Kucharski J. 2010. Activity of β-glucosidase, arylsulfatase and phosphatases in soil contaminated with copper. J. Elem. 15(1): 213-226.

Słowa kluczowe:

copper, β-glucosidase, phosphatase, arylsulfatase, resistance rate, soil contamination with copper

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