Formation of adenylate energy charge (AEC) versus the fluorine content in forest soil in the area affected by emission from Police Chemical Plant


Wydanie: 2 / 2010

Autorzy:

  • Telesiński A., Smolik B., Grabczyńska E.

Kategorie: Biology and microbiology

DOI: 10.5601/jelem.2010.15.2.355-362

Abstrakt:

Activity of most enzymes of the key-metabolic pathways depend on the content of adenine nucleotides, such as ATP, ADP and AMP, in cells. Based on the level of these nucleotides, the adenylate energy charge (AEC) was defined as AEC = ([ATP] + 0.5[ADP]) : ([ATP]+ [ADP] + [AMP]). Theoretically, AEC values can range from 0 to 1 and represent the physiological state of a soil microbial population. Soil microorganisms and the enzymes they secrete are connected with biological processes which form soil fertility in all ecosystems, including forests. Forests are such ecological systems which are an integral complex and their dysfunction could be caused by permanent influence of anthropogenic factors, including industrial emission of gases and dusts.The aim of this study was the determination of changes in adenylate energy charge values and fluorine content in the humus layer of forest-podsol soils affected by the emission of Police Chemical Plant. During a year, soil samples were taken five times (in October 2007, February, April, June and September 2008) from five different sites Wkrzańska Forest near Węgornik, Tatynia, Tanowo, Trzeszczyn and Mścięcino. In the samples, concentration of fluorine, both water-soluble (extracted by 0.01 M CaCl2) and potentially accessible to plants (extracted by 2 M HClO4), was assayed by potentiometry. Additionally, the content of adenine nucleotides was assayed by chromatography. Based on the content of nucleotides, adenylate energy charge values in soil were calculated. The AEC values and fluorine content in soil depended on a distance from the emitter and the dates onwhich the samples were taken. In order to determine the relationships between the fluorine content and AEC values, Pearson’s correlations coefficients were calculated. Between the fluorine (both, water-soluble and plant available) content and AEC values there was a signifficant negative correlation, which could mean that AEC is a very good indicator of the fluorine content in soil.


Cytacja:

Telesiński A., Smolik B., Grabczyńska E. 2010.  Formation of adenylate energy charge (AEC) versus the fluorine content in forest soil in the area affected by emission from Police Chemical Plant. J. Elem. 15(2): 355-362.

Słowa kluczowe:

fluorine, adenylate energy charge, soil, forest

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