The study was carried out to asses the influence of soil mineral fertilization, manure application and soil liming on changes in the concentration of microelements (B, Cu, Fe, Mn and Zn) in soil solution. The tests were performed on samples of sandy soil collected from long-term fertilization experiments being conducted on lessive soil of the granulometric structure of clayish sand. Soil solution was prepared with the suction method. The concentration of microelements in soil solution was determined by means of the ICP method. The results showed an increase in Fe, Mn, Zn and Cu and a decrease in B and Mo in soil solution when soil was fertilized with nitrogen. The concentration of microelements in soil solution was not significantly influenced when soil was fertilized with phosphorous and potassium. Liming had a significant effect on a decrease in the concentration of Fe, Mn, Zn and Cu and an increase in B and Mo in soil solution. Soil application of manure significantly increased the concentration of microelements in soil solution.